翻译 弥漫大B细胞淋巴瘤细针穿刺标本中的CD10、BCL6和MUM1表达

译者：邱燕华【关注儿童】江西省儿童医院病理科啊癌症—细胞病理学杂志背景：基因表达谱将弥漫大B细胞淋巴瘤（DL...






译者：邱燕华【关注儿童】江西省儿童医院病理科啊

癌症—细胞病理学杂志

背景：基因表达谱将弥漫大B细胞淋巴瘤（DLBCL）分为两种主要类型：生发中心B细胞（GCB）型和非生发中心B细胞（non-GCB）型。这种分类用免疫组化方法检测特异性抗体如CD10、BCL6和MUM1具有很好的重复性。细针穿刺在非霍奇金淋巴瘤的诊断中起着重要的作用，而且一些病例可能只有通过细针穿刺才能获取到病理标本。本研究的目的是评估CD10、BCL6和MUM1在将细针穿刺标本制作成细胞块和传统涂片中的免疫细胞化学染色差异，并与组织学结果进行比较。

方法：对38例细针穿刺诊断的DLBCL标本进行研究。这38例标本，22例制作成细胞蜡块，16例进行传统涂片，相应的所有病例进行CD10、BCL6和MUM1染色。这些结果与24例组织学切片染色结果进行比较。

结果：免疫细胞化学染色成功33例（18例细胞块和15例传统涂片），5例染色不成功（4例细胞块和1例传统涂片）。根据CD10、BCL6和MUM1表达情况，33例DLBCL中GCB型9例、非GCB型24例。免疫细胞化学染色结果与24例组织学切片染色结果一致。

结论：CD10、BCL6和MUM1可以用于细针穿刺标本的免疫细胞化学染色。本实验结果证实通过细胞块和传统涂片进行免疫细胞化学染色是可行的，并且与免疫组织化学染色结果具有同等效果。

CD10, BCL6, and MUM1 expression in diffuse large B-cell lymphoma on FNA samples.

Cozzolino I,Varone V,Picardi M,Baldi C,Memoli D,Ciancia G,Selleri C,De Rosa G,Vetrani A,Zeppa P

Cancer Cytopathology； February 2016 Volume 124, Issue 2:135-43

Gene expression profiling has divided diffuse large B-cell lymphoma (DLBCL) into 2 main subgroups: germinal center B (GCB) and non-GCB type. This classification is reproducible by immunohistochemistry using specific antibodies such as CD10, B-cell lymphoma 6 (BCL6), and multiple myeloma oncogene 1 (MUM1).

Fine-needle aspiration (FNA) plays an important role in the diagnosis of non-Hodgkin lymphoma, and in some cases FNA may be the only available pathological specimen. The objectives of the current study were to evaluate CD10, BCL6, and MUM1 immunostaining on FNA samples by testing the CD10, BCL6, and MUM1 algorithm on both FNA cell blocks (CB) and conventional smears (CS), evaluating differences in CB and CS immunocytochemical (ICC) performance, and comparing results with histological data.

Thirty-eight consecutive DLBCL cases diagnosed by FNA were studied. Additional passes were used to prepare CB in 22 cases and CS in 16 cases; the corresponding sections and smears were immunostained using CD10, BCL6, and MUM1 in all cases. The data obtained were compared with histological immunostaining in 24 cases.

ICC was successful in 33 cases (18 CB and 15 CS) and not evaluable in 5 cases (4 CB and 1 CS). The CD10-BCL6-MUM1 algorithm subclassified DLBCL as GCB (9 cases) and non-GCB (24 cases). ICC data were confirmed on histologic staining in 24 cases.

CD10, BCL6, and MUM1 ICC staining can be performed on FNA samples. The results herein prove it is reliable both on CB and CS, and is equally effective and comparable to immunohistochemistry data. Cancer (Cancer Cytopathol) 2016;124:135-143. © 2015 American Cancer Society.

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