甘油三酯试剂测定

业力生物小喇叭——E1014甘油三酯（油脂、食品）酶法测定试剂盒

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甘油三酯（油脂、食品）酶法测定试剂盒 E1014

试剂盒优势：试剂盒避免了有毒的氯仿甲醇提取、繁杂的氮气吹干和脂质复溶等步骤，采用高效能试剂进行裂解和甘油三酯抽提，以及优化的GPO Trinder酶学反应组分和操作步骤。简单易行，灵敏度高！

E1014105 assays750元描述：高油脂食品材料，如液态油脂、油料植物组织和种子、脂肪组织、肌肉间脂肪等的甘油三酯测定远比与血液甘油三酯测定复杂。试剂盒避免了有毒的氯仿甲醇提取、繁杂的氮气吹干和脂质复溶等步骤，采用高效能试剂进行裂解和甘油三酯抽提，以及优化的GPO Trinder酶学反应组分和操作步骤。简单易行，灵敏度高，线性范围20~2000 µmol/L。适用于农业、畜牧业、食品材料，用于动物脂肪肌肉、植物叶片种子、土壤、以及固态食品材料、含高浓度油料样品中甘油三酯的精确测量。

适于测定样品
(1) 动物脂肪组织、肌间脂肪
(2) 油料植物组织和种子如花生大米等
(3) 固态食品材料、土壤
(4) 液态成品油或含高浓度油脂的样品
其它类型的组织细胞甘油三酯测定用E1013；血液、培养液甘油三酯测定使用E1003。

测定原理：根据经典GPO Trinder反应，脂肪酶分解血甘油三酯为甘油。甘油激酶催化甘油生成3-磷酸甘油，后者被磷酸甘油氧化酶氧化生成过氧化氢，在过氧化物酶催化下进行生色反应，在550nm有最大吸收峰，光密度值与甘油三酯浓度成正比。

组成 (105次微板测定或30次1 ml比色杯测定)
(1) 裂解液 100 ml
(2) R1试剂 16 ml
(3) R2试剂 4 ml
(4) 4 mmol/L甘油标准品1 ml
4 ºC避光储存3月。

所需设备：酶标仪、生化分析仪或721、722型可见光分光光度计。96孔微板或比色杯光径1cm。最佳工作波长550nm，如无此波长建议优先选用570nm、次选530、490nm。

部分发表论文：
Zhang Xuequn, Yang Juntao, Guo Yuanbiao, Ye Hua, Yu Chaohui, Xu Chengfu, Xu Lei, Wu Songfeng, Sun Wei, Wei Hangdong, Gao Xue, Zhu Yunping, Qian Xiaohong, Jiang Ying, Li Youming, He Fuchu, 2009. Functional proteomic analysis of nonalcoholic fatty liver disease in rat models: Enoyl–coenzyme a hydratase down-regulation exacerbates hepatic steatosis. Hepatology, 51(4): 1190-1199. IF: 10.88

Luo Zhidan, Ma Liqun, Zhao Zhigang, He Hongbo, Yang Dachun, Feng Xiaoli, Ma Shuangtao, Chen Xiaoping, Zhu Tianqi, Cao Tingbing, Liu Daoyan, Nilius Bernd, Huang Yu, Yan Zhencheng, Zhu Zhiming, 2011. TRPV1 activation improves exercise endurance and energy metabolism through PGC-1α upregulation in mice. Cell Research, doi:10.1038/cr.2011.205. IF: 9.416

Pang Shanshan, Tang Haiqing, Zhuo Shu, Qin Zang Ying, Le Yingying, 2010. Regulation of Fasting Fuel Metabolism by Toll-Like Receptor 4. Diabetes, 59(12): 3041-3048. IF: 8.889

Ding Yunfeng, Yang Li, Zhang Shuyan, Wang Yang, Du Yalan, Pu Jing, Peng Gong, Chen Yong, Zhang Huina, Yu Jinhai, Hang Haiying, Wu Peng, Yang Fuquan, Yang Hongyuan, Steinbuchel Alexander, Liu Pingsheng, 2011. Identification of the major functional proteins of Prokaryotic lipid droplets. The Journal of Lipid Research, doi:10.1194/jlr.M021899. IF: 6.114

Zhao Zhigang, Luo Zhidan, Wang Peijian, Sun Jing, Yu Hao, Cao Tingbing, Ni Yinxing, Chen Jing, Yan Zhencheng, Liu Daoyan, Zhu Zhiming, 2011. Rosiglitazone Restores Endothelial Dysfunction in a Rat Model of Metabolic Syndrome through PPARγ- and PPARδ-Dependent Phosphorylation of Akt and eNOS. PPAR Research, doi:10.1155/2011/291656. IF: 2.726

Deng Jiagang, Li Chunyang, Wang Hailian, Hao Erwei, Du Zhengcai, Bao Chuanhong, Lv Jianzhen, Wang Yi, 2011. Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells. Biochemical and Biophysical Research Communications, 411(3): 523–529. IF: 2.593

Zhai, W., Xu, C., Ling, Y., Liu, S., Deng, J., Qi, Y., Londos, C., Xu, G., 2010. Increased Lipolysis in Adipose Tissues is Associated with Elevation of Systemic Free Fatty Acids and Insulin Resistance in Perilipin Null Mice, Hormone Metabolic Research, 42: 247-253. IF: 2.414

Yang Zhi, Yin Ji-Ye, Gong Zhi-Cheng, Huang Qiong, Chen Hao, Zhang Wei, Zhou Hong-Hao, Liu Zhao-Qian, 2009. Evidence for an effect of clozapine on the regulation of fat-cell derived factors. Clinica Chimica Acta, 408(1–2): 98-104. IF: 2.388

He Yong-Han, He Ying, Liao Xi-Lu, Niu Yu-Cun, Wang Guan, Zhao Chen, Wang Liang, Tian Ming-Jie, Li Ying, Sun Chang-Hao, 2012. The calcium-sensing receptor promotes adipocyte differentiation and adipogenesis through PPARγ pathway. Molecular and Cellular Biochemistry, 361(1-2): 321-328. IF: 2.167

Ju Dapeng, He Jingjing, Zhao Lili, Zheng Xueli, Yang Gongshe, 2011. Estrogen related receptor α-induced adipogenesis is PGC-1β-dependent. Molecular Biology Reports, 39(3): 3343-3354, DOI: 10.1007/s11033-011-1104-8. IF: 1.875